Biotinyl Human IGF-I

Biotinyl human insulin-like growth factor-I (biotinyl IGF-I) is made by reacting human IGF-I with Sulfo-NHS-Biotin reagent to label the primary amines of the IGF-I molecule. A mixture of biotinylated forms are produced, the major species being di-biotinyl IGF-I. Biotinyl IGF-I has been prepared with a spacer to minimize steric hindrance during subsequent avidin binding.