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Human LR³ insulin-like Growth Factor-I (LR³IGF-I) was developed by GroPep Bioreagents specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR³IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR³IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules, which are responsible for promoting cell survival by inhibition of apoptosis.This LR³IGF-I Rapid™ ELISA kit combines GroPep’s expertise in the field of IGF research and Biosensis’ newly established Rapid™ ELISA platform. This has resulted in the new LR³IGF-I Rapid™ ELISA kit, which provides for the sensitive, specific and reliable quantification of LR³IGF-I in less than 3 hours. The ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR³IGF-I in culture media. Included in the kit are mouse monoclonal anti- LR³IGF-I capture antibody pre-coated onto an ELISA plate, standard LR³IGF-I protein, a biotinylated anti- LR³IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of LR³IGF-I present in samples and the supplied protein standard. This LR³IGF-I Rapid™ ELISA kit has been developed, optimized and validated to quantify LR³IGF-I in cell culture medium. It is likely to be used to measure LR³IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
THE ASSAY SHOULD BE VALIDATED FOR SPECIFIC PURPOSE IN THE USER LABORATORY